欢迎访问今晚必中四不像图片!证券代码:871574
首页 成 果 源于西力产品的客户论文
Tandemly duplicated CYP82Ds catalyze 14-hydroxylation in triptolide biosynthesis and precursor production in Saccharomyces cerevisiae. Nat. Commun., 2023, 14: 875.
发布时间:2023-08-10 阅读数:1870 来源:原创
分享到:

Abstract:

Triptolide is a valuable multipotent antitumor diterpenoid in Tripterygium wilfordii, and its C-14 hydroxyl group is often selected for modifification to enhance both the bioavailability and antitumor effificacy. However, the mechanism for 14-hydroxylation formation remains unknown. Here, we discover 133 kb of tandem duplicated CYP82Ds encoding 11 genes on chromosome 12 and characterize CYP82D274 and CYP82D263 as 14-hydroxylases that catalyze the metabolic grid in triptolide biosynthesis. The two CYP82Ds catalyze the aromatization of miltiradiene, which has been repeatedly reported to be a spontaneous process. In vivo assays and evaluations of the kinetic parameters of CYP82Ds indicate the most signifificant affifinity to dehydroabietic acid among multiple intermediates. The precursor 14-hydroxy-dehydroabietic acid is successfully produced by engineered Saccharomyces cerevisiae. Our study provides genetic elements for further elucidation of the downstream biosynthetic pathways and heterologous production of triptolide and of the currently intractable biosynthesis of other 14-hydroxyl labdane-type secondary metabolites.


今晚必中四不像图片

ewm.jpg 微信公众号
11.jpg 移动官网
特别声明:禁止在未经同意情况下转载本网站信息,BioBioPha品牌产品均由今晚必中四不像图片独家生产与销售,仅用于科学研究或企业研发!
版权所有 2019-2024 今晚必中四不像图片 BioBioPha 滇ICP备09000810号-1滇公安备案号 53019002000069号
技术支持:奥远科技

联系客服